Naslov
Comparison of inflammatory markers in healing of exisional wounds in db/db and healthy C56BL/6 mice

Autori
Šitum, Kristina ; Glojnarić, Ines ; Brajša, Karmen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinica Chimica ACTA / Rainbow Sandra ; Hallworth Mike - Lahti : ISSN 0009-8981, 2005

Skup
16th IFCC-FESCC European Congress of Clinical Biochemistry and Laboratory Medicine-EUROMEDLAB 2005

Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 08.05.2005. - 12.05.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
wound healing; SAA; db/db mice

Sažetak
Inflammation, as initial phase of wound healing, is accompanied by a variety of physiological and biochemical changes whose are collectively called the acute phase response. Inflammatory reaction occurs immediately at the wound site and inflammatory mediators cause also the systemic changes. Genetically diabetic mice (db/db mice) are useful as an animal model for wound-healing studies, since wound healing in these animals is markedly delayed as compared to non-diabetic littermates. Differences in systemic inflammatory response during cutaneous wound repair between db/db mice and their littermate controls could enable us to evaluate whether the attenuation of the systemic inflammatory response is also delayed in db/db mice.In this study, serum amyloid A protein (SAA), the most sensitive systemic inflammatory marker in mice [1-3], was quantified during closure of 8 mm excisional wounds induced in the back skin of healthy C57Bl/6 and db/db mice, at days 3, 6, 9 and 13 after wounding, and its levels were compared to hematological parameters (total WBC, neutrophil and lymphocyte percentage).In healthy C57Bl/6 mice, SAA concentration declined significantly from day 3 to day 6 after the wound excision while in db/db mice SAA concentration declined from day 6 to day 9 after the wounding revealing the prolongation of the wound healing process in db/db mice. Comparison of SAA and hematology measurements also provided the evidence of faster attenuation of systemic inflammation related to the wound closure process in healthy C57Bl/6 than in db/db mice. Since hematological analyses provided unclear results, we found SAA measurements more appropriate for monitoring of inflammation during the wound-healing process in both healthy and diabetic mice.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA