Naslov
Apoptosis of pancreatic B-cells in alloxan treated rats: p53 immunohistochemical study

Autori
Veljača, Marija ; Brajša, Karmen ; Glojnarić, Ines ; Ševeljević-Jaran, D. ; Krnić, Žarka ; Ferenčić, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Abstracts of EUROTOX 97 : Diversification in Toxicology : man and environment u: Pharmacology and toxicology. Suplement (ISSN 0901-9936) 80 (1997) (S3) / - , 1997, 177-177

Skup
EUROTOX 97 : Diversification in Toxicology : man and environment

Mjesto i datum
Århus, Danska, 25.07.1997. - 28.07.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pancreatic B-cells; apoptosis; p53

Sažetak
Althrough alloxan has been widely used as an experimental diabetogen, alloxan induced apoptosis of pancreatic B-cells was not described until recently. The aim of this study was to investigate weather p53, tumor suppressor protein, and "the guardian" of genome is involved in apoptosis induction after alloxan challenge. A single sc administration of 150 mg/kg of alloxan monohydrate to female Wistar rats resulted in severe hyperglycemia together with clinical features of diabetes. Histological analysis revealed significant arte of apoptosis in HE-stained, paraffin embedded pancreatic section 48 h after alloxan treatment, in contrast to no apoptosis in control rats. Immunohistochemical staining performed by p53 (Ab-7) kits (Onkogene, Calbiochem), showed significant p53 expression as early as 15 min after alloxan administration, lasting 24 h, no staining in controls. the disapperance of p53 might have been involved in triggering of cell dying by apoptosis in alloxan treated rats.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA