Naslov
Agrobacterium-mediated transformation of some conifers

Autori
Mihaljević, Snježana ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Slovenian Symposium on Plant Physiology with International Participation, Gozd Martuljek, Slovenia, 29 Sept - 1 Oct 1993 / - , 1993

Skup
1st Slovenian Symposium on Plant Physiology with International Participation

Mjesto i datum
Gozd Martuljek, Slovenija, 29.09.1993. - 01.10.1993

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pinus nigra; Sequoia sempervirens; Agrobacterium; rooting; transformation

Sažetak
Vegetative propagation of most conifer species is slow by traditional methods, mostly because of their difficulty in forming roots. Genes beared by agrobacterial Ri plasmid, known to induce root formation when transferred into herbaceous, could be used to transform woody species in the sense of promoting root regeneration in their cuttings. Explants of Pinus nigra (black pine) and Sequoia sempervirens (redwood) were used in this investigation. Two-month-old epicotyl explants of black pine and 1-2 cm length shoots of redwood produced in vitro, clone CA3, were infected on basal cut with the wild strain A. rhizogenes 8196 or transconjugant strain A. tumefaciens bearing Ri plasmid C58C1(pArA4abc). 24 hours after inoculation explants were rinsed in carbenicillin solution (1g/L) and cultured on 1/2xGD medium (black pine) or 1/2xMS medium (sequoia), supplemented with 2 % sucrose and 500 mg/L carbenicillin. Adventitious roots were formed in 20-100 % of explants (1-3 per explant), eight to ten weeks after inoculation and showed native root phenotype. Both strains were capable of inducing root regeneration. The presence of Ri plasmid (pRi8196) in regenerated roots was confirmed by the mannopine content. Physical conditions of culture (solid or liquid medium, activated charcoal addition) and season influenced the appropriate development of the induced roots.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA