Naslov
4-Chloroindole-3-acetic and indole-3-acetic acids in Pisum sativum

Autori
Magnus, Volker ; Ozga, Jocelyn A. ; Reinecke, Dennis M. ; Pierson, Gerald L. ; Larue, Thomas A. ; Cohen, Jerry D. ; Brenner, Mark L.

Izvornik
Phytochemistry (0031-9422) 46 (1997), 4; 675-681

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Pisum sativum; Fabaceae; garden pea; hormone analysis; free and conjugated auxin; 4-chloroindole-3-acetic acid; IAA

Sažetak
Endogenous IAA and 4-chloroindole-3-acetic acid (4-Cl-IAA) were analyzed in vegetative and reproductive tissues of the garden pea (Pisum sativum L.) using GC-MS selected ion monitoring in the presence of stable-isotope labeled internal standards. In fruit collected 3 - 8 days after anthesis (DAA) conjugates of both auxins were more abundant than the free hormones. Auxin levels (ng/g fr. wt) in the seeds were higher by 1 - 2 orders of magnitude than in the pericarps. However, as seeds are small at this stage, the pericarp nevertheless contains a substantial fraction of the overall quantity of IAA and 4-Cl-IAA. While, in young fruit tissues, IAA was more abundant than 4-Cl-IAA, the opposite was true for seeds at the table-ready stage and for roots of nine-day-old seedlings. Our data suggest that both IAA and 4-Cl-IAA are required to coordinate the vegetative and reproductive growth of pea plants.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA