Naslov
Effect of Bgl2p and Ccw12p Mannoproteins on Stability of Yeast Cell Wall

Autori
Teparić, Renata ; Barbarić, Slobodan ; Mrša, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3. hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista / Marić, Vladimir ; Colić-Barić, Irena ; Glancer-Šoljan, Margareta - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 1998, 148-148

Skup
3. hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista

Mjesto i datum
Zagreb, Hrvatska, 10.06.1998. - 12.06.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
S. cerevisiae cell wall; yeast mannoproteins

Sažetak
The cell wall of the yeast S. cerevisiae contains a number of mannoproteins and for most of them physiological function is unknown. Mannoproteins are either loosely bound to the wall and can be extracted by SDS, or strongly bound and can be extracted only upon glucanase treatment, suggesting covalent bounding to glucan in the cell wall. Most abundant detergent-extractable protein is Bgl2p protein, while Ccw12p is major protein among those strongly bound to the wall. Bgl2p protein shows endoglucanase and glucosyltransferase activity in vitro, indicating a role of this protein in the cell wall glucan assembly, while Ccw12p protein is not characterized yet. In order to assess possible physiological role of those proteins, mutant strains are constructed by disruption of BGL2 or CCW12 genes. Sensitivity of the cell wall to beta-1, 3-glucanase treatment was examined and both mutant strains had increased sensitivity in comparison to the parental strains, showing that these proteins contribute somehow to cell wall stability. However, transformation of mutant strains with episomal plasmid containing CCW12, results in reversion of wild type level of glucanase sensitivity in ccw12, but not in the bgl2 strain. This shows that two proteins affect cell wall stability by different mechanisms. In addition, it was found that overexpression of CCW12 in the wild type cells did not cause increased stability of the cell wall.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA