Naslov
Morphometrical analysis and needle volatiles composition of some hard pine species and their hybrids

Autori
Idžojtić, Marilena

Izvornik
Glasnik za šumske pokuse (0352-3861) 38 (2001); 1-76

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Pinus nigra J. F. Arnold; P. densiflora Siebold et Zucc.; P. thunbergiana Franco; interspecific hybrids; needle morphology; needle anatomy; shoot tracheids; discriminant analysis; volatiles; GC; GC/MS; terpenes; cluster analysis

Sažetak
The paper deals with nineteen morphological and anatomical traits of needles and shoots of three pine species (Pinus nigra J. F. Arnold, P. densiflora Siebold et Zucc. and P. thunbergiana Franco) and their four hybrids (F1 hybrids P. nigra × P. densiflora ; reciprocal hybrids P. densiflora × P. nigra ; F1 hybrids P. nigra × P. thunbergiana, and reciprocal hybrids P. thunbergiana × P. nigra). The analysed traits are as follows: needle length, fascicle sheath length, one-year shoot tracheid length und width, the number of ventral and dorsal stomatal rows, the number of stomata along one row, the number of serrations along one needle margin ; needle cross-section area, needle cross-section hight and diameter ; stellar region cross-section area ; stellar region cross-section height ; stellar region cross-section diameter ; the largest number of hypodermal cell layers on the needle cross-section ; the number of medial resin canals on needle cross-section ; the largest and the smallest number of sheath cells surrounding a single resin canal. The possibility to diferentiate the hybrids from the parent species was investigated. Based on the analysed traits, with a probability range of between 95% and 100%, it was possible to discriminate F1 hybrids P. nigra × P. densiflora, P. densiflora × P. nigra, and P. thunbergiana × P. nigra from the parent species. Hybrids P. nigra × P. thunbergiana differ significantly from the male parent, the Japanese black pine, but they do not differ from the female parent, the European black pine. The features, by which it is possible to identify the hybrids, were separated by a discriminant analysis. Chemical analytical methods (gas chromatography and gas chromatography/mass spectrometry) were used to determine, in terms of quality and quantity, the composition of needle volatile of the mentioned species and hybrids. Cluster analysis was used to determine to what degree the individual species and hybrids resemble in terms of the composition of the needle volatile. The Japanese black pine and its hybrids (P. nigra × P. thunbergiana and P. thunbergiana × P. nigra) differ greatly from other species and hybrids. The second unit consists of two groups. The first is composed of the European black pine and the hybrids P. nigra × P. densiflora. The second group consists of the Japanese red pine and the hybrids P. densiflora × P. nigra. The proportion of alpha-pinene is the largest of all components in the needle volatile of all analysed species and hybrids (between 25.8% in P. densiflora and 42.7% in P. nigra), except for the Japanese black pine in which the largest proportion is that of beta-pinene (34.1%). The component which is specific of the European black pine is germacene D, the one specific of the Japanese red pine is thunbergol, while beta-pinene is specific of Japanese black pine. In F1 hybrids the proportion of the individual components is larger, smaller or mostly intermediary between the respective proportions of the parent species. The following are the components whose proportions in all analysed F1 hybrids are intermediary between the respective proportions of the parent species: alpha-pinene, beta-burbonene, beta-caryophyllene, germacrene D and alpha-muurolene. These components could be used for the verification of F1 hybrid plants.

Izvorni jezik
Engleski

Znanstvena područja
Šumarstvo

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