Naslov
The expression of EBV markers and CD30 molecule in the lymph nodes during infectious mononucleosis

Autori
Vince, Adriana ; Jeren, Tatjana

Izvornik
Clinical microbiology and infection (1198-743X) 6 (2000), Suppl.1;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
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Sažetak
Objective: To analyze the morphologic changes, EBV and CD30 antigen expression in the lymph nodes during EBV mononucleosis by fine needle aspiration (FNA). Patients and methods: The FNA of the enlarged neck lymph nodes was performed in 70 patients with EBV mononucleosis. Cytomorphological changes were analyzed by using May Grünwald-Giemsa stain. The expression of the antigens ; ZEBRA, EBNA-2 and CD30 was visualized by the immunocytochemical LSAB (labelled streptavidin avidin, DAKO) method. Results: Transformed lymphocytes account for 12-65% of all cells in the aspirates (an average of 30%). Small lymphocytes account for 60% and plasma cells 7.5%. Follicle center cells were rarely found in the FNAs. Reactive Reed Sternberg (RS) cells were detected in 27% of FNA specimens epitheloid cells in only 9%. Diagnostic suspicion of malignant lymphoma could not be eliminated by cytological analysis in 3 (4.3%) patients. Lytic antigen ZEBRA was positive on 0.5% lymphatic cells ; EBNA-2 on 0.1%. All transformed lymphocytes as well as reactive RS cells were CD30 negative, while the latter ones were also negative to the analyzed EBV markers. Conclusion: Transformed lymphocytes are the most dominant feature in the lymph nodes during acute EBV mononucleosis. The presence of many transformed lymphocytes (immunoblasts) as well as reactive Reed-Sternberg cells can raise the suspicion towards malignant lymphoma (immunoblastic or Hodgkin). However all reactive cells were CD30 negative, which can be used ad diagnostic criteria in morphologically doubtful cases. EBV markers are expressed on 1:1000 lymphocytes, suggesting a small number of infected B-cell, which proves the fact that the lymphoproliferation is mostly caused by a strong T-lymphocyte response.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA