Naslov
Mechanism of liquefaction of human ejaculate. I. Changes of the ejaculate proteins

Autori
Koren, Eugen ; Lukač, Josip

Izvornik
Journal of reproduction and fertility (0022-4251) 56 (1979); 493-499

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ejaculate proteins ; ejaculate enzymes

Sažetak
The spontaneous liquefaction of the human ejaculate in vitro was studied by macroscopic inspection, disc electrophoresis of the ejaculate proteins, free amino acid and nitrogen determinations and by electron microscopy. Liquefaction occurred in three phases. Phase 1 was characterized by the macroscopic solubilization of the gelatinous material and by disappearance of its granular ultrastructure. Zn++, Hg++ and Cu++ completely inhibited the first step of the liquefraction, which could be reversed by Na2EDTA. The factor(s) involved is most probably a proteolytic enzyme(s) which is tightly incorporated into the gelatinous material. In Phase 2 of liquefaction solubilized proteins were degraded to peptides and in Phase 3 these peptides were degraded to amino acids. Factor(s) responsible for these two steps of liquefaction could be removed by washing the gelatinous material.

Izvorni jezik
Engleski

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