Pregled bibliografske jedinice broj: 189259
The metalloenzymic nature of collagenase-like peptidase from rat testis
The metalloenzymic nature of collagenase-like peptidase from rat testis // Journal of reproduction and fertility, 49 (1977), 95-99 doi:10.1530/jrf.0.0490095 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 189259 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The metalloenzymic nature of collagenase-like
peptidase from rat testis
Autori
Lukač, Josip ; Koren, Eugen
Izvornik
Journal of reproduction and fertility (0022-4251) 49
(1977);
95-99
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
metalloenzymes ; collagenase-like peptidase
Sažetak
Collagenase-like peptidase, an enzyme degrading synthetic collagenase substrate (PZ-pentapeptide), was purified from rat testes and its properties were examined. Its activity was strongly inhibited by chelating agents, such as EDTA and 1, 10- phenanthroline. By chelation and exhaustive dialysis it was possible to obtain this enzyme in its inactive, metal-free form. The activity of the metal-free enzyme was partly recovered by treatment with zinc or manganese ions, while a combined zinc and manganese treatment resulted in complete recovery of enzyme activity.
Izvorni jezik
Engleski
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
