Naslov
The effect of collagenase-like peptidase from rat testis and clostridial collagenase A on the rat seminal vesicle secretion and its coagulation

Autori
Koren, Eugen ; Lukač, Josip ; Milković, Stevan

Izvornik
Journal of reproduction and fertility (0022-4251) 36 (1974); 161-167

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
collagenase-like peptidase ; rat seminal vesicle secretion

Sažetak
Collagenase-like peptidase (CLP), an enzyme recently discovered in human, rat and bull spermatozoa and rat and bull testes, was purified from the rat testis homogenate by acetone precipitation and gel filtration on Sephadex G- 200. This enzyme is inhibited by cysteine and EDTA, but not by DFP and Trasylol, and does not show measurable non-specific proteolytic or amidase activity. Using disc electrophoresis on polyacrylamide gels, it was demonstrated that purified CLP from rat testis and clostridial collagenase A degraded the basic proteins, and to a lesser extent some of the neutral and acid proteins from the rat seminal vesicle secretion. A certain similarity was observed in their basic protein degradation products. It was ascertained by macroscopical examination that CLP and clostridial collagenase A prevent, by their lytic action, coagulation of the vesicular secretion. This applies both to spontaneous and vesiculase- mediated coagulation. The specificity of collagenases and the rôle of CLP are discussed.

Izvorni jezik
Engleski

POVEZANOST RADA