Naslov
Loss of heterozygosity of APC and DCC tumor suppressor genes in human sporadic colon cancer

Autori
Šturlan, Sanda ; Kapitanović, Sanja ; Kovačević, Duje ; Lukač, Josip ; Spaventi, Šime ; Spaventi, Radan ; Pavelić, Krešimir

Izvornik
Journal of molecular medicine (0946-2716) 77 (1999), 3; 316-321

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Colon cancer; Tumor suppressor genes; APC; DCC; Loss of heterozygosity
(colon cancer; tumor suppressor genes; APC; DCC; loss of heterozygosity)

Sažetak
We examined 36 cases of human sporadic colon carcinoma and corresponding normal tissue samples to evaluate loss of heterozygosity at the APC and DCC tumor suppressor genes loci using restriction fragment length polymorphism polymerase chain reaction and variable nucleotide tandem repeat analysis. Observed informativity was 83% for APC and 75% for DCC. DNA from 6 (20%) of 30 informative tumors exhibited loss of heterozygosity at the APC locus. Loss of heterozygosity at the DCC locus was observed in 7 (26%) of 27 informative tumor DNAs. Our results support the view that malignant progression is a consequence of more than one genetic change and suggest that inactivation of APC and DCC genes plays a role in a multistep process of colon tumor progression.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA