Naslov
Identification of Human Papilloma Virus (HPV) Genotypes in Human Placenta Using Non-Radioactive In Situ Hybridization Method

Autori
Pirkić, Ahmed ; Grubišić, Goran ; Herman, Radoslav ; Košec, Vesna ; Vukosavić-Cimić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of First European Congress of Colposcopy and Cervical Pathology / British Society for Colposcopy and Cervical Pathology - Dublin : University College Dublin, 1998, 112-112

Skup
First European Congress of Colposcopy and Cervical Pathology

Mjesto i datum
Dublin, Irska, 24.09.1998. - 26.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
genotipovi HPV; posteljica; non-radioaktivna insitu hibridizacija
(HPV genotypes; human placenta; non-radioactive insitu hybridization)

Sažetak
The aim of this stuidy was to demonstrate poresence of HPV genomes in placentas of pregnant women in whom genital warts before pregnancy had been observed clinically and colposcopically. Histpathological and immunohistochemical analysis of the warts specimens confirmed cervical HPV infection. Tissue specimens of seven placentas were examined for HPV DNA genomes using three "cocktails" of the biotinilated HPV DNA probes specific to 6/11, 16/18 and 31/33/51 types ("Enzo Diagnostics"). In 3 of 7 placentas 6/11 and 31/33/51 types of the HPV DNA were demonstrated. Hybridization signal of various intensity was predominantly of the "dot" type, and it was observed in villous cytotrophoblast, syntitial gigantocytes, and in the extravillous trophoblast cells which penetrated the vascular wall of the basal plate. Demonstrated presence of HPV DNA in placentas of pregnant women with genital warts indicates possibility of extension of the cervical HPV infection to the fetus.

Izvorni jezik
Engleski

POVEZANOST RADA