Naslov
Liposomal gel with chloramphenicol: characterization and in vitro release

Autori
Pavelić, Željka ; Škalko-Basnet, Nataša ; Jalšenjak, Ivan

Izvornik
Acta pharmaceutica (1330-0075) 54 (2004), 4; 319-330

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
liposomes; chloramphenicol; bioadhesive gel; stability; vaginal application

Sažetak
The aim of our study was to develop a liposomal carrier system for the local treatment of bacterial vaginosis, capable to efficiently deliver entrapped drug during an extended period of time. Chloramphenicol was entrapped in liposomes composed of egg phosphatidylcholine/egg phosphatidylgycerol-sodium (9/1, molar ratio) and prepared by two different methods ; the proliposome and the polyol dilution method, respectively. Both liposome preparations were characterised and compared for particle size, polydispersity, entrapment efficiency and tested for in vitro stability in media that simulate human vaginal conditions (buffer, pH 4.5 and vaginal fluid simulant). To achieve application viscosity of liposomes and to further improve their stability, liposomes prepared by the proliposome method were incorporated in the bioadhesive gel made of Carbopol 974P NF resin. In vitro release studies of liposomes incorporated in the gel have shown a slower release of entrapped chloramphenicol. Even after 24 hours of incubation in the vaginal fluid stimulant more than 40 % of the originally entrapped drug was still retained in the gel. Storage stability studies have proved the ability of the Carbopol 974P NF gel to preserve original size distributions of incorporated liposomes. All the performed experiments confirm the applicability of liposomes as a novel drug carrier system for the local treatment of bacterial vaginosis.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA