Naslov
Positive regulation of ERK activation and MKP-1 expression by peroxovanadium complex bpV (phen)

Autori
Rumora, Lada ; Hadžija, Mirko ; Maysinger, Dusica ; Žanić-Grubišić, Tihana

Izvornik
Cell biology and toxicology (0742-2091) 20 (2004); 293-301

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
apoptosis ; mitogen-activated protein kinase ; mitogen-activated protein kinase phosphatase-1 ; peroxovanadium

Sažetak
Lower micromolar concentrations of peroxovanadium compound potassium bisperoxo(1, 10-phenanthroline)oxovanadate (V)  bpV (phen) stimulate RINm5F cell metabolic activity. 1 and 3  M bpV (phen) induces strong and sustained activation of extracellular signal-regulated kinase (ERK). However, it seems that bpV (phen) does not effect c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) phosphorylation. In addition, bpV (phen) induces mitogen-activated protein kinase phosphatase-1 (MKP-1) expression. We found that ERK activation could be completely abolished if RINm5F cells were incubated with both bpV (phen) and PD 98059, a specific inhibitor of upstream ERK kinase MEK1. On the other hand, this combined treatment up-regulated activation of stress kinases, JNK and p38 MAPK, significantly suppressed MKP-1 expression and induced cell death. Thus, our results suggest that the mechanism underlying bpV (phen) survival-enhancing effect could be associated with induced ERK activation and MKP-1 expression.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

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