Naslov
Cytokines in LPS mediated immunity against Klebsiella infection

Autori
Vasiljev, Vanja ; Rukavina, Tomislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
"Biotechnology and Immuno-modulatory Drugs" Programme and Abstracts / Croatian Society of Biotechnology - Zagreb : Medicinska naklada, 2005, 35-35

Skup
4th Croatian Scientific Conference on Biotechnology - "Biotechnology and Immuno-Modulatory Drugs"

Mjesto i datum
Zagreb, Hrvatska, 20.02.2005. - 23.02.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Klebsiella pneumoniae; proinflammatory cytokines; anti-inflammatory cytokine

Sažetak
In the assasment of immunity to the encapsulated virulent strain of Klebsiella pneumoniae Caroli we used monoclonal antibody against O1 LPS. The survival rate of animals protected with monoclonal antibody after the lethal intraperitoneal challege with 5 times LD50 was 70% while all animals in control group died within four days after infection. In this study we examined the production of proinflammatory (IL-1b, IL-6, IL-12, IFN-g and TNF-a) and anti-inflammatory (IL-10) cytokines between animals protected with monoclonal antibody and control group of animals. Concentrations of individual cytokines from plasma were determined by ELISA. We found that almost all proinflammatory cytokines in the control group reached their maximal values 24 hours after infection while in the protected group concentrations were significantly lower. On the contrary, at specific time point IL-10 concentration was significantly higher in the protected group. We concluded that such cytokine pattern in the protected group was responsible for the survival of these animals.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA