Naslov
Phylogenetic relationships within the genus Orobanche as revealed by RAPD analysis

Autori
Roman, Belen ; Alfaro, Carmen ; Torres, Ana Maria ; Moreno, Maria Teresa ; Šatović, Zlatko ; Pujadas, Antonio ; Rubiales, Diego

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka 8. hrvatskog biološkog kongresa s međunarodnim sudjelovanjem / Besendorfer, Višnja - Zagreb : Hrvatsko biološko društvo, 2003, 41-43

Skup
8. Hrvatski biološki kongres s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 27.09.2003. - 02.10.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Orobanche; RAPD; phylogenetic relationships; genetic diversity

Sažetak
RAPD markers were used to study variation among 51 populations belonging to 20 taxa in the genus Orobanche: O. alba, O. amethystea, O. arenaria, O. ballotae, O. cernua, O. clausonis, O. cumana, O. crenata, O. densiflora, O. foetida, O. foetida var. broteri, O. gracilis, O. haenseleri, O. hederae, O. latisquama, O. mutelii, O. nana, O. ramosa, O. rapum-genistae and O. santolinae. A total of 202 amplification products generated with five arbitrary RAPD primers was obtained and species-specific markers were identified. Distance matrices and dendrograms were generated using Dice coefficient and neighbor-joining cluster analysis. The pattern of interspecific variation obtained is in general agreement with previous taxonomic studies based on morphology, and the partition into two different sections (Trionychon and Orobanche) is generally clear. However, the position in the dendrogram of O. clausonis did not fit this classification since it clustered with members of section Trionychon. Within this section, O. arenaria was relatively isolated form the other members of the section: O. mutelii, O. nana and O. ramosa. Within section Orobanche, all O. ramosa populations showed a similar amplification pattern, whereas differences among O. crenata populations growing on different hosts were found. O. foetida and O. densiflora clustered together, supporting the morphological and cytological similarities and the host preferences of these species.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

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