Naslov
Effect of nicardipine and amlodipine on the brain free arachidonic acid level in hypoxic rats

Autori
Župan, Gordana ; Eraković, Vesna ; Mršić, Jasenka ; Varljen, Jadranka ; Simonić, Ante ; Milin, Čedomila

Izvornik
Periodicum biologorum (0031-5362) 98 (1996), 1; 107-110

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Hypoxia ; calcium channel blockers ; brain free arachidonic acid rats

Sažetak
Background and purpose: A number of investigations support the hypothesisthat a common denominator of hypoxic cellular damage in the brain is an increase in intracellular CA2+ concentration that causes degradation of membrane phospholipids and liberation of cerebral free fatty acids, particularly free arachidonic acid (FFA). The aim of our study was to examine the effects of the calcium channel blockers, nicardipine and amlodipine, on the level of the brain FAA in rats exposed to hypoxia. Material and methods: The study was carried out on Hannover-Wistar rats. The drugs tested had been injected 30 minutes prior to hypoxia exposure. Rats were decapitated 15 minutes after losing the righting reflex. The brains of all animals were taken out and were frozen in liquid nitrogen. FAA was quantified using gas chromatography method. Results: The results of our experiments show that an accumulation of the brain FAA occurs during the period of posthypoxic normoxic reoxygenation in hypoxia-exposed rats. The tested doses of nicardipine and amplodipine did not influence the increase in the brain FAA level in hypoxic rats. Conclusions: The inhibition of the CA2+ influx via L-type of voltage-sensitive calcium channels is not sufficient for the attenuation of the release of the brain FAA in hypoxic rats.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA