Naslov
Manipulating mammalian genome by gene targeting

Autori
Volarević, Siniša ; Pende, Mario ; Pullen, Nicholas

Izvornik
Croatian medical journal (0353-9504) 40 (1999); 368-374

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
genetic engineering; knockout mice; genetic recombination

Sažetak
The development of strategies which allow the inactivation of specific murine genes by homologous recombination in embryonic cells has revolutionized biological science in the last 10 years. A large number of mice carrying genetic lesions, generated by gene targeting technology, has tremendously increased our knowledge in many areas of biology, culminating in the identification of mouse models for human genetic disorders. These findings have been recently complemented by "conditional" gene targeting technology, allowing gene inactivation in a defined tissue and at a specific time point during development or adulthood, thereby extending the sophistication and potential of this technology.

Izvorni jezik
Engleski

POVEZANOST RADA