Naslov
Human papillomaviruses DNA infection in head and neck squamous cell carcinomas detected by dot-blot hybridization

Autori
Cerovac, Željka ; Lučin, Ana ; Šarčević, Božena ; Kralj, Zvonimir ; Ban, Jasna

Izvornik
Periodicum biologorum (0031-5362) 100 (1998), 4; 527-535

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Ljudski papiloma virusi ; tumori glave i vrata ; točkasta hibridizacija ; histološki stupnjevi
(HPV ; head and neck carcinomas ; histological grade ; dot-blot hybridization)

Sažetak
Human papillomaviruses (HPV) infection could be associated with the development of some human tumors of squamous epithelium. Twenty one squamous cell carcinomas (8 larynx, 3 tongue, 6 oral cavity and 4 pharynx), with different grading, were analyzed for the presence of HPV DNA by dot-blot hybridization under the stringent conditions with digoxigenin labelled probes for HPV types 6, 16 and 18. Positive HPV signals were found in DNA isolated from 10 invasive carcinomas (47.6%), 4 (19%) epithelia adjacent to carcinomas, and 2 (11.7%) resected margins. HPV-positive signals were obtained in 44% of oral cavity and tongue, in 62.5% of laryngeal, and in 25% of pharyngeal carcinomas. Out of 10 HPV- positive tumors a single HPV type was determined in 80%, and double infection in 20% cases. As the only type HPV 6 DNA was determined in two (20%), HPV 16 DNA in 5 (50%), and HPV 18 DNA in 1 (10%) head and neck carcinoma. The detection rate of HPV 6 was lower than of HPV 16 or 18 for all tumor sites. Among all tumors HPV DNA was determined in 57% histologically graded as well differentiated, 20% as moderately differentiated, and 55% as poorly differentiated head and neck carcinomas. HPV DNA sequences are determined in 47.6% of analyzed head and neck tumors. The presence of HPV in these tumors suggests their potential etiological role in the development and progression of head and neck carcinomas.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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