Pregled bibliografske jedinice broj: 18460
A microplate assay for DNA damage determination (Fast Micromethod) in cell suspensions and solid tissues
A microplate assay for DNA damage determination (Fast Micromethod) in cell suspensions and solid tissues // Modern aspects in monitoring of environmental pollution in the sea / Muller, Werner E.G. (ur.).
Mainz: Akademie Gemeinnutziger Wissenschaften zu Erfurt, 1998. str. 35-39 (predavanje, nije recenziran, cjeloviti rad (in extenso), znanstveni)
CROSBI ID: 18460 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
A microplate assay for DNA damage determination (Fast Micromethod) in cell suspensions and solid tissues
Autori
Batel, Renato
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Modern aspects in monitoring of environmental pollution in the sea
/ Muller, Werner E.G. - Mainz : Akademie Gemeinnutziger Wissenschaften zu Erfurt, 1998, 35-39
Skup
Modern aspects in monitoring of environmental pollution in the sea
Mjesto i datum
Mainz, Njemačka, 07.11.1997. - 08.11.1997
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Nije recenziran
Ključne riječi
DNA damage; fast micromethod
Sažetak
A rapid and convinient procedure for the determination of DNA damage in cell suspensions and solid tissues on single microplates was developed. The procedure is based on the ability of commercially available fluorochromes (e.g. picogreen) to interact preferentially with dsDNA in the presence of ssDNA, RNA and proteins at high ph (> 12.0), thus allowing direct measurements of DNA denaturation without any sample handling or stepwise DNA separation
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA