Pregled bibliografske jedinice broj: 183522
Detection of Herpes simplex virus by real-time PCR
Detection of Herpes simplex virus by real-time PCR // 4. hrvatski kongres medicinskih biokemičara s međunarodnim sudjelovanjem : knjiga sažetaka
Zadar, Hrvatska, 2003. str. 13-13 (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 183522 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Detection of Herpes simplex virus by real-time PCR
Autori
Kozić, Sanja ; Vince, Adriana ; Iščić-Beš, Janja ; Baća-Vrakela, Ivana ; Kutela, Nataša
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
4. hrvatski kongres medicinskih biokemičara s međunarodnim sudjelovanjem : knjiga sažetaka
/ - , 2003, 13-13
Skup
Hrvatski kongres medicinskih biokemičara s međunarodnim sudjelovanjem (4 ; 2003)
Mjesto i datum
Zadar, Hrvatska, 24.09.2003. - 28.09.2003
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
herpes simplex virus; real-time; PCR
Sažetak
Herpes simplex viruses types (HSV) 1 and 2 cause a variety of clinical symptoms in the central nervous system (CNS). HSV infection often occurs in immunocompromised patients and in neonates leading to serious complications and death. A molecular detection of HSV DNA became a reference standard assay for CNS infections caused by HSV. Aim was tThe implementation of sensitive, specific and rapid laboratory test for detection of HSV DNA and differentiation of HSV 1 and HSV 2. A total of 50 cerebrospinal fluid (CSF) samples taken from patients with encephalitis or meningoencephalitis were analyzed. DNA was extracted from CSF by rapid extraction protocol. The HSV DNA detection was performed by newly developed LightCycler (LC) instrument that enables a real-time detection of PCR products (continuous monitoring of amplicon development). Detection was based on the fluorescence resonance energy transfer (FRET) principle. Melting curve analysis enabled distinguishing HSV 1 and HSV 2 strains. Quality control was assured by using internal controls (IC), negative and positive controls. By LC PCR we have detected 4 HSV 1 positive samples out of 50. Two positive patients were newborns, and two were adults. None of the samples was HSV 2 positive. Real-time PCR is sensitive, specific and rapid method for detection of HSV DNA in CSF samples. Analysis may be done in less than two hours, what is essencial for early therapy initiation.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
