Naslov
Increased numbers of CD38 molecules on bright CD8+ T-lymphocytes in acute infections with Epstein-Barr virus, cytomegalovirus and Human Immunodeficiency Virus

Autori
Židovec-Lepej, Snježana ; Brkić, L ; Vince, Adriana ; Đaković-Rode, Oktavija ; Remenar, A ; Kutela, Nataša ; Jeren, Tatjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual Meeting of the Croatian Immunological Society 2003 / - , 2003

Skup
Annual Meeting of the Croatian Immunological Society 2003

Mjesto i datum
Brijuni, Hrvatska, 17.10.2003. - 19.10.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
EBV; CMV; HIV

Sažetak
Objective: The aim of this study was to quantify the expression of CD38 on CD8+ T-lymphocytes of patients with infectious mononucleosis caused by Epstein-Barr Virus (EBV) and Cytomegalovirus (CMV) as well as in acute Human Immunodeficiency Virus type 1 (HIV-1) infection. In order to investigate the possible diagnostic usefulness of this molecule, we compared the levels of CD38 expression in persons with typical and atypical clinical and/or laboratory presentation of EBV-induced mononucleosis. Patients and methods: The study enrolled 19 persons with typical clinical and laboratory parameters compatible with EBV-induced infectious mononucleosis, 10 persons with atypical clinical presentation of this disease (absence of lymphadenopathy n=1, maculopapular skin rash not related to antibiotic use n=1, autoimmune haemolytic anaemia n=1, lymphocytosis higher than 30x109/L white blood cells N=4, only a few "reactive" lymphocytes in the peripheral blood n=3). In addition, 13 persons with acute CMV infection, 5 persons with acute HIV-1 infection and 15 healthy controls were also included in the study. Quantitative flow cytometry was performed by CELLQUANT CD38/CD8 kit (Biocytex, France). Results: All groups of persons with infectious mononucleosis (Atypical presenatation of acute EBV infection and CMV-infected persons) had significantly increased CD38 expression compared to healthy controls (median 556 CD38 molecules per CD8+ T-cell, range from 350 to 988, p<0, 001 for three groups). Median number of CD38 molecules per CD8+ T-cell in patients with typical clinical presentation of EBV-induced infectious mononucleosis was 52 095 molecules perCD8+ T-cell (range from 35 403 to 87 109) and it was not significantly different from patients with atypical presentation of disease (median of 49672 CD38 molecules per CD8+ T-cell, range from 24 900 to 86395, p=0, 71). The expression of CD38 in patients with CMV-induced infectious mononucleosis (median 25 420 CD38 molecules per CD8* T-cell, range 13 252 to 37 262) was significantly lower compared to both groups patients with EBV-induced infectious mononucleosis (p 0, 001). The expression of CD38 on CD8+ T-cells in all five parsons with acute HIV-1 infection (from 39 560 to 55 326 CD38 molecules per CD8+ T-cell) was similar to those with EBV-induced infectious mononucleosis. Conclusion: Acute infections with EBV, CMV and HIV-1 significantly increase CD38 expression on CD8+ T-cells. CD38 quantification could be helpful in differential diagnostics of atypical clinical and/or laboratory presentation of EBV-induced infectious mononucleosis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA