Naslov
Macromolecular prodrugs : VI. kinetic study of poly[alpha, beta-(N-2-hydroxyethyl-DL-aspartamide)]-ketoprofen hydrolysis

Autori
Jakšić, Planinka ; Mlinarić-Majerski, Kata ; Zorc, Branka ; Dumić, Miljenko

Izvornik
International journal of pharmaceutics (0378-5173) 135 (1996), 1-2; 177-182

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
polymeric prodrug; ketoprofen; poly[alpha; beta-(n-2-hydroxyethyl-dl-aspartamide)]; poly[alpha; beta-(n-2-hydroxyethyl-dl-aspartamide)]-ketoprofen conjugate; hydrolysis; enzyme; high performance liquid chromatography

Sažetak
The kinetics of hydrolysis of ketoprofen from poly[alpha, beta-(N-2-hydroxyethyl-DL-aspartamide)]-ketoprofen conjugate (PHEA-Ket) in aqueous buffer solutions in the pH range 1.0-10.6 at 37 degrees C was studied. The hydrolysis of PHEA-Ket conjugate followed the pseudofirst-order kinetic. The following rate constants were obtained: k = 1.542 x 10(-2)h (-1) at pH = 1.0, k = 6.260 x 10(-3) h(-1) at pH = 9.2, and k = 0.155 h(-1) at pH = 10.6. At pH = 5.0 and 7.0 no significant hydrolysis was observed. The PHEA-Ket was also stable after exposure to several enzymatic systems in neutral medium. Determination of ketoprofen was performed by high performance liquid chromatography using diclofenac as the internal standard.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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