Naslov
EFFECTS OF VANADATE ON OLEIC ACID INDUCED INSULIN RESISTANCE IN CULTURED RAT HEPATOCYTES

Autori
Roša, Jagoda ; Roša, Josip

Izvornik
Diabetologia Croatica (0351-0042) 33 (2004), 1; 17-21

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
amino acid transport; anti-diabetic; insulin; insulin resistance; oleic acid; protein kinase C; vanadate

Sažetak
The aim of this study was to elucidate mechanism of oleic acid induced insulin resistance and vanadate action on amino acid transport in cultured rat hepatocytes. Hepatocytes were isolated by a modified collagenase perfusion technique and cultured for 24 h in M199 serum-free medium. To assess amino acid transport, hepatocytes were incubated in Hanks-Hepes medium containing ?-amino14C-isobutyric acid (AIB) to the final concentration of 1 mmol/L. In hepatocytes obtained from rats on standard diet insulin almost two fold increased AIB transport (5.57ą0.31 vs. 10.15ą0.21 ; p<0.01). Oleic acid did not change basal AIB transport but reduced insulin effect to less than a half value (5.36ą0.27 vs. 6.25ą0.19 ; p<0.01). Vanadate increased basal (5.57ą0.3 vs. 10.97ą0.35 ; p<0.01) and did not change insulin stimulated (10.15ą0.21 vs. 11.23ą0.45 ; N.S.) AIB transport in normal hepatocytes. It normalized insulin-stimulated transport in oleic acid-induced resistant hepatocytes (5.36ą0.27 vs. 11.25ą0.19 ; p<0.01), but not in TPA treated ( 6.82ą0.2 vs. 6.7ą0.25 ; N.S.) resistant cells. Vanadate is able to prevent and reverse insulin resistance induced by oleic acid not involving of protein kinase C.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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