Naslov
Cytological changes in callus cultures of Allium commutatum Guss.

Autori
Pavlica, Mirjana ; Pevalek-Kozlina, Branka

Izvornik
Phyton (0079-2047) 39 (1999), 3; 301-304

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Allium commutatum Guss.; callus culture; cytology

Sažetak
Callus tissue was induced on root tips of in vitro cultured seedlings of Allium commutatum Guss. cultured on MS medium supplemented with 4.6 mM kinetin and 4.5 mM 2, 4-dichlorophenoxyacetic acid (2, 4-D). Developed calli were transferred to the same basal medium with addition of 1.0, 2.5 and 5.0 mM 2, 4-D or without 2, 4-D. After three weeks in culture adventitious shoot and root induction was observed in compact greenish coloured callus on medium without 2, 4-D. On media supplemented with 1.0 and 2.5 mM 2, 4-D the callus was compact and yellowish with adventitious roots. No organogenesis was noticed on medium with 5.0 mM 2, 4-D and callus tissue was whitish and slimy. For preliminary cytological analyses samples of callus tissue were collected on the 7. and 14. day after the transfer. Fixed calli fragments were hydrolized in 1 N HCl at 60 °C for 11 minutes and stained in toto with basic fuchsin. The slides were prepared according to standard Feulgen squash technique. In each sample 1 000 cells were screened for mitotic activity and for establishing the rate of abnormalities. Relatively low level of genetic alterations was found. Among them micronuclei and polyploid cells were observed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA