Naslov
Alfpha-1-antitrypsin in serum of patients with COPD

Autori
Malić, Ana ; Dodig, Slavica ; Grdić, Marija ; Plevnik, Helena ; Ruljančić, Nedjeljka ; Čepelak, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress Of The Croatian Society Of Biochemistry And Molecular Biology (HDBMB 2004) : abstracts / Dumić, Jerka - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2004

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation

Mjesto i datum
HOC Bjelolasica, Hrvatska, 30.09.2004. - 02.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
alfpha-1-antitrypsin; COPD; oxidative stress

Sažetak
α -1-antitrypsin (α -1-AT) is a protective protein in human plasma and after penetration into all body fluids and tissues it protects structural proteins from degradation. Lungs are most dependent upon protective action of α -1-AT because they are most exposed to infections, and the antineutrophil elastase activity of α -1-AT seems to have a homeostatic function. Proteolytic destruction of the lung parenchyma, resulting in emphysema and loss of lung elastic recoil, leads to chronic obstructive pulmonary disease (COPD). α -1-AT deficiency is the one of known genetic risk factor for COPD, just as well oxidative stress regarding the fact that cigarette smoking is the major risk factor for this disease. So, protease-antiprotease balance along with oxidant-antioxidant balance play a key role in pathogenesis of COPD because of a complex interaction between genetic and environmental factors. The aim of our study was to determinate α -1-AT concentration in serum of patients with COPD (n=23) in comparison with healthy subjects (n=57 ; smokers n=23, non-smokers=34). α -1-AT concentrations are determinated using radial immunodiffusion and immunoturbidimetric method. There were no significant deference between healthy smokers and non-smokers ( for smokers α -1-AT concentration was 1, 82± ; ; ; 0, 63 g/L, and for non-smokers it was 1, 75± ; ; ; 0, 51 g/L) and the both were in reference range of 1, 4 – 3, 5 g/L. α -1-AT concentrations in patients were 2, 30± ; ; ; 0, 88 g/L and therefore 28% higher than in healthy subjects, although in reference range. To conclude, protease-antiprotease balance in our patients with COPD remained unchanged in comparison to healthy subjects. Concerning that α -1-AT is a protein of acute phase we can assume that concentrations α -1-AT in our study show an adaptive answer to inflammatory that is still in process.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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