Naslov
Catalytic activity of GGT in patients with COPD

Autori
Grdić, Marija ; Malić, Ana ; Dodig, Slavica ; Kulušić, Marija ; Čepelak, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress Of The Croatian Society Of Biochemistry And Moleculam Biology (HDBMB 2004) : abstracts / Dumić, Jerka - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2004

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation

Mjesto i datum
HOC Bjelolasica, Hrvatska, 30.09.2004. - 02.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
GGT activity; glutathione; COPD

Sažetak
Chronic obstructive pulmonary disease (COPD) is a complex condition characterized by airflow limitation that is not fully reversible. Oxidative stress is the most important feature in COPD because the foremost cause of COPD is smoking and atmospheric pollution. Glutathione (GSH) is important intra and extracellular antioxidant in the lungs. It protects the lung cells against oxidants, electrophilic compounds, and xenobiotics that cause oxidative stress. GSH is synthetased in cells during reaction catalyzed by two enzymes  -glutamylcysteine synthetase and GSH synthetase. Synthetased GSH becomes a part of GSH redox system, which uses GSH for detoxification in cell. Part of GSH that leaves the cell is broken down by   glutamyl transpherase(GGT), this enzyme breaks the   glutamyl bond of GSH and release glutamic acid which is then transferred into the cell where it is used for the synthesis of new GSH. It is clearly that GGT is involved in synthesis of GSH, and through that, in indirect way in protection against oxidative stress. The aim of this study was to investigate if there is a difference between the activity of GGT in serum of healthy volunteers and patients with diagnosed COPD. The measurement of the GGT activity was conducted on a group of 58 healthy volunteers (21 non smokers, 17 subjects who have quit smoking and 20 smokers), and a group of 20 patients with COPD. Measurement of the GGT was conducted by using IFCC enzymatic method at 37 C. The mean value of the GGT activity for each group was calculated and the results were: non-smokers 44.26 ± ; ; ; 29.95, former smokers 40.13 ± ; ; ; 28.33, smokers 40.95 ± ; ; ; 18.04, patients with COPD 28.60 ± ; ; ; 15.03 U/L. GGT activity in the group of the healthy volunteers was higher in comparison with the group of the patient with COPD, while the GGT activity between subgroups of the healthy volunteers was not significantly different. To determine how that decreased GGT activity influences on the pathogenesis and development of COPD further investigation is needed.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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