Naslov
Validation of a Method for Chloramphenicol Residue in Muscle Tissue

Autori
Kubala-Drinčić, Helena ; Bažulić, Davorin ; Sapunar-Postružnik, Jasenka ; Grubelić, Mirela ; Stuhne, Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CERMM-3 3rd Central European Conference on Reference Materials and Measurements, Book of Abstracts / - , 2002

Skup
CERMM-3 3rd Central European Conference on Reference Materials and Measurements

Mjesto i datum
Rogaška Slatina, Slovenija, 30.05.2002. - 01.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chloramphenicol; gas chromatography; matrix solid phase dispersion; muscle tissue

Sažetak
A method for gas chromatographic (GC) determination of chloramphenicol (CAP) residues in muscle tissue was developed and validated. Extraction of CAP residues was performed by the technique of matrix solid-phase dispersion (MSPD). The final extract was evaporated and the trimethylsilyl derivative of CAP was detected by GC-ECD and GC-MS. For quantification, the internal standard used was the meta isomer of CAP (m-CAP) for GC-ECD. The method was validated by determining accuracy (recovery of CAP from fortified muscle tissue samples and analysis of the certified reference material BCR-445), repeatability and detection and quantification limits. Mean recoveries were 93, 96 and 98% at the levels of 5, 10 and 15 μ g CAP/kg, respectively. The repeatability at the same levels was 13, 11 and 3%, respectively. The limits of detection and quantification with ECD were 1.6 μ g CAP/kg and 4.0 μ g CAP/kg, respectively.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA