Naslov
Rapid determination of glucose in food samples by an amperometric glucose-sensing electrode

Autori
Grabarić, Zorana ; Tićac, Dunja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
2nd Central European Meeting 5th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists

Mjesto i datum
Opatija, Hrvatska, 17.10.2004. - 20.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
glucose biosensor; amperometry; food analysis

Sažetak
Of the various methods available for determination of glucose in industrial food laboratories the most attractive are those based on amperometric enzyme electrodes. The combination of specific enzyme with sensitive electrochemical signal transduction yields enzyme electrodes, which provide simple, rapid and accurate determination of enzyme substrate. In this work cross-linking glutaraldehyde-BSA procedure for immobilization of enzyme was used. The enzyme was immobilized on the surface of a spectroscopically pure carbon electrode modified electrochemically with the layer of potassium nickel hexacyanoferrate (FeNiHCM). Glucose determinations were performed at – 50 mV vs. saturated calomel electrode Hg&frac12 ; ; Hg2Cl2&frac12 ; ; 3M KCl in 0.1 M KCl and phosphate buffer (pH = 5.5) as well as in mixed solvents (ethanol/water). The results obtained indicate that solid graphite electrode was successfully modified with the catalytic layer of FeNiHCM. Amperometric response of biosensor was pH and organic solvent volume fraction dependent. Linear concentration range from 0.1 mM to 1.00 mM glucose was obtained. In various samples of food such as yogurt (Bioaktiv LGG, Dukat), creams with chocolate (Mus, Dukat and Paradies Cream, Dr. Oetker) and strawberry flavour (Ledeni desert, Dr. Oetker) and whip cream powder (Šlag pjena, Aro) glucose was successfully determined.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA