Naslov
Increased mortality of Saccharomyces cerevisiae cell wall protein mutants

Autori
Teparić, Renata ; Stuparević, Igor ; Mrša, Vladimir

Izvornik
Microbiology (1350-0872) 150 (2004), 10; 3145-3150

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cell wall proteins; Pir proteins; Scw proteins

Sažetak
Yeast cell wall contains unusually high number of different mannoproteins. Physiological role of most of them is unknown and gene disruptions leading to depletion of different proteins did not affect major functions of the wall. In this work phenotype of different single and multiple cell wall protein mutants is observed at the level of individual cells. It was found that the lack of noncovalently bound wall proteins Scw4p, Scw10p and Bgl2p increases the mortality of S. cerevisiae cells grown logarithmically under standard laboratory conditions, as assayed by methylene blue staining. Mutation of SCW11, however, suppresses the phenotype of scw4 scw10, or scw4 scw10 bgl2 indicating that Scw4p, Scw10p and Bgl2p act synergistically while Scw11p has an activity antagonistic to that of the other three proteins. The mutants lacking major covalently bound proteins, either all four described Pir-proteins, or 5 most abundant GPI-anchored proteins (Ccw12p, Ccw13p/Dan1p, Ccw14p/Icwp1p, Tip1p and Cwp1p) also had increased mortalities, the first somewhat more and the latter less than that of scw4 scw10 bgl2. In all cases the observed phenotype was suppressed by the addition of osmotic stabiliser to the growth medium indicating that cells died due to decreased osmotic stability. If cells were grown to the stationary phase, Scw-mutants showed only slightly increased mortality but mutants lacking Pir-, or GPI-anchored proteins had significantly increased sensitivity suggesting that their physiological function is primarily expressed in stationary cells. In many cases structures consisting of a living ccw5 ccw6 ccw7 ccw8 (multiple Pir-protein mutant) mother with two methylene blue-stained daughters could be seen.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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