Pregled bibliografske jedinice broj: 176562
Application of molecular methods to nosocomial epidemiology Example:Investigation of Pseudomonas aeruginosa outbreak on hematology ward by pulse field gel electrophoresis
Application of molecular methods to nosocomial epidemiology Example:Investigation of Pseudomonas aeruginosa outbreak on hematology ward by pulse field gel electrophoresis // Fifth congress of the International Federation of Infection Control : abstracts
Zagreb, 2004. str. 31-32 (pozvano predavanje, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 176562 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Application of molecular methods to nosocomial epidemiology Example:Investigation of Pseudomonas aeruginosa outbreak on hematology ward by pulse field gel electrophoresis
Autori
Plečko, Vanda ; Kalenić, Smilja ; Budimir, Ana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Fifth congress of the International Federation of Infection Control : abstracts
/ - Zagreb, 2004, 31-32
Skup
Congress of the International Federation of Infection Control (1 ; 2004)
Mjesto i datum
Poreč, Hrvatska, 09.10.2004. - 12.10.2004
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
molecular typing; Pseudomonas aeruginosa
Sažetak
Strain typing is a technique used to identify organisms that share same phenotypic and genotypic traits and that are then assumed to be clonally related (i.e.genetically identical or closely related to each other).Strain typing facilitates identification of nosocomial outbreak and modes of transmission. To be useful, a typing system should have typeability, reproducibility and high discriminatory power. Pulsed field gel electophoresis (PFGE) is a method that separates large chromosomal DNA fragments (digested with low-frequency restriction endonucleases). These large DNA fragments are resolved with PFGE, in which the electrical fields are altered, enabling the resolution of large DNA molecules. On hematolgy ward in CHC Zagreb in year 2003/2004, emerged multiresistant Pseudomonas aeruginosa . There were isolates from blood (22 patients) and surveillance cultures (9). We have collected strains and performed PFGE. The aim of this workshop is to interpretate results of PFGE according to typeability, reproducibility and discrminatory power and to compare visual and computer assisted interpretation of pilsotypes. Also, criteria for clonality relatedness and implementation of PFGE results with other methods used for outbreak control will be discussed.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
