Pregled bibliografske jedinice broj: 175277
Fluorescence microscopy for detecting mycoplasmas in cell cultures
Fluorescence microscopy for detecting mycoplasmas in cell cultures // Proceedings of 6th Multinational Congress on Microscopy, European Extension
Pula, Hrvatska, 2003. str. 376-377. (poster, međunarodna recenzija, cjeloviti rad (in extenso), ostalo)
CROSBI ID: 175277 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Fluorescence microscopy for detecting mycoplasmas in cell cultures
Autori
Busch, K. ; Herak-Perković, V. ; Tudja, M. ; Dvoraček, L. ; Kriljac, Z.
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), ostalo
Izvornik
Proceedings of 6th Multinational Congress on Microscopy, European Extension
/ - , 2003, 376-377.
Skup
6th Multinational Congress on Microscopy
Mjesto i datum
Pula, Hrvatska, 01.06.2003. - 05.06.2003
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
florescence microscopy; detection of mycoplasma; DNA staining method; cell cultures
Sažetak
In this study sensibility of florescence microscopy in detection of mycoplasma in cell cultures was established. Twenty cell culture samples were examined by direct method (modified Hayflick, s medium was used for cultivation) and indirect DNA staining method. Both methods revealed the presence of Mycoplasma sp. in 8 samples, and in 12 samples the findings were negative. Mycoplasma hyorhinis ATCC 1798 and Mycoplasma orale ATCC 23714 were positive controls. Only indirect staining method with bis-benzimide-DNA complex revealed both positive controls. Mycoplasma orale ATCC 23714 was detected by direct and indirect methods. Only indirect method showed the growth of positive Mycoplasma hyorhinis ATCC 1798.
Izvorni jezik
Engleski
Znanstvena područja
Kemijsko inženjerstvo
