Naslov
A thin chip microsprayer system coupled to Fourier transform ion cyclotron resonance mass spectrometry for glycopeptide screening
(A thin chip microsprayer system coupled to Fourier transform ion cyclotron resonance mass spectrometry for glycopeptide screening)

Autori
Bindila, Laura ; Froesch, Martin ; Lion, Niels ; Vukelić, Željka ; Rossier, Joël S. ; Girault, Hubert H. ; Peter-Katalinić, Jasna ; Zamfir, Alina D.

Izvornik
RCM. Rapid communications in mass spectrometry (0951-4198) 18 (2004), 23; 2913-2920

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
thin chip microsprayer system ; FTICR mass spectrometry ; glycopeptide screening

Sažetak
A thin polymer microchip was coupled with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FT-ICR MS) at 9.4 T and the method was optimized in negative ion mode for glycopeptide screening. The interface between the polymer microchip and FT-ICR MS consists in an in-laboratory conceived and designed mounting system that exhibits robustness and performant alignment of the chip toward the MS inlet. The particular attribute of the polymer chip coupled to FT-ICR MS to achieve an increase of ionization efficiency and sensitivity under the premise of high mass accuracy of detection is highlighted by a large number of major and minor glycopeptide structures detected and identified in highly heterogeneous mixtures obtained from urine matrices. Glycoforms expressing various saccharide chain lengths ranging from tri- to dodecasaccharide bearing up to three sialic acid moieties could be detected and according to their molecular ions assigned under the accuracy of an average mass deviation below 6 ppm. The (-)thin chipESI/FT-ICR MS is a potent novel system for glycomic screening of complex mixtures, as demonstrated for identification of single sialylated O-glycosylated amino acids and peptides from urine matrices and could be considered for general applicability in glycoanalytical field.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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