Naslov
Erythropoietic changes in rats after 2.45 GHz nonthermal irradiation

Autori
Busljeta, Ivana ; Trosic, Ivancica ; Milkovic-Kraus, Sanja

Izvornik
International Journal of Hygiene and Environmental Health (1438-4639) 207 (2004); 1-6

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
rats; whole-body irradiation; nonthermal; RF/MW fields; anuclear cells; erythropoietic precursor cells; peripheral blood parameters

Sažetak
The purpose of this study was to observe the consequence on erythropoiesis of rats subchronically exposed to radiofrequency microwave (RF/MW) irradiation at nonthermal level. Adult male Wistar rats (N=40) were exposed to 2.45 GHz continuous wave RF/MW fields for 2 hours daily, 7 days weekly, at power density 5-10 mW/cm2. Exposed animals were divided into four subgroups (n=10 animals in each subgroup) in order to be irradiated for 2, 8, 15 and 30 days. Animals were euthanized on the final irradiation day of each treated subgroup. Sham-exposed rats were used as control ones (N=24). Six animals were included into the each control subgroup. Bone marrow smears were examined to determine absolute counts of anuclear cells and erythropoietic precursor cells. The absolute erythrocyte count, haemoglobin and haematocrit values were observed in the peripheral blood by an automatic cell counter. The bone marrow cytogenetic analysis was accomplished by micronucleus (MN) test. On the irradiation days 8 and 15, erythrocyte count, haemoglobin and haematocrit were increased in peripheral blood. Concurrently, parameters in the bone marrow, i.e. anuclear cells and erythropoietic precursor cells were decreased but micronucleated cells frequency was increased. In the applied experimental condititon, RF/MW radiation might cause disturbance in red cell maturation and proliferation, and induce micronucleus formation within erythropoietic cells.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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