Naslov
Protein co-factor of yeast seryl-tRNA synthetase

Autori
Ročak, Sanda ; Godinić, Vlatka ; Landeka, Irena ; Weygand-Đurašević, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8. Hrvatski biološki kongres / Besendorfer, Višnja - Zagreb : Hrvatsko biološko društvo, 2003, 75-75

Skup
8. Hrvatski biološki kongres

Mjesto i datum
Zagreb, Hrvatska, 27.09.2003. - 02.10.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
tRNASer ; seryl-tRNA synthetase ; peroxin

Sažetak
Peroxin Pex21p was identified as seryl-tRNA synthetase-interacting protein in Saccharomyces cerevisiae both in vivo and in vitro. Besides its main function in peroxisome biogenesis, Pex21p acts as specific activator of SerRS by increasing the efficiency of aminoacylation. In order to map SerRS/Pex21p interactive domains, several deletion mutants of SerRS have been produced. The interaction with peroxin was almost entirely lost after deleting positively charged C-terminal peptide of SerRS, which characterizes all eukariotyc seryl-tRNA synthetases. We have previously shown that such truncation also leads to toxicity in vivo, due to altered substrate recognition. These results suggest the involvement of Pex21p in stabilizing the conformation of SerRS active site, which could be important for maintaining the accuracy of serylation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA