Naslov
Effects of atrazine toxicity on androgen metabolism in rat

Autori
Šimić, Branimir ; Zechner-Krpan, Vesna ; Kniewald, Zlatko ; Kniewald, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1. skup Hrvatskog društva za biotehnologiju / Hrvatsko društvo za biotehnologiju - Zagreb : Hrvatsko Društvo za Biotehnologiju, 1998

Skup
1. skup Hrvatskog društva za biotehnologiju

Mjesto i datum
Zagreb, Hrvatska, 19.02.1998. - 20.02.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
atrazine; testosterone metabolism; pituitary; prostate; rat

Sažetak
Biological effectiveness of androgen hormones depends on their biosynthesis, as well asd on the metabolism in target and peripheral tissues. It is well known that various xenobiotics can interfere with physiological processes within the reproductive system. The aim of this study was to give more insight in the possible harmful effects of s-triazine herbicide atrazine (A) on androgen mechanisms. The metabolism of testosterone (T) in rat pituitary and prostate after in vitro addition of 0,232-0.696 micromol A. or after the oral treatment with A in daily dose of 120 mg/kg bw for 7 days, was investigated. Atrazine inhibited in vitro the activity of 5-alpha-reductase (5-alpha-R), and it caused an augmentation of the activity of 3-alpha-hydroxysteroid dehydrogenase (3-alpha-HSD) in the pituitary of prepubertal rat. In vivo, treatment with A augmented the conversion of T into androstenedione (D), and further into 5-alpha-androstanedione (5-alpha-D). An increase if 5-alpha-R activity was found 7 days following cessation of the treatment. In sexually mature rat the conversion of T into D was inhibited, but the activities of 5-alpha-R and of 3-alpha-HSD were augmented on the 1st day after the termination of A treatment. On the 7th post-treatment day the conversion of T into 5-alpha-dihydrotestosterone (DHT) was inhibited. In the prostate A inhibited the activity of 5-alpha-R in vitro. The conversion of T into D and further into 5-alpha-D was stimulated under the in vitro and in vivo influence of A. Atrazine exerted the combined antigonadotropic and antiandrogenic properties in male rat. The antiandrogen-like action has been expressed as the primary effect of the treatment: the changes of T metabolism in the pituitary, the decrease in the weight of seminal vesicles and the increase of prostate weight, and as we published previously (1) the inhibition of specific DHT-receptor complex formation in rat prostate cytosol. The antigonadotropic activity has been shown during the post-treatment period: the decrease of pituitary 5-alpha-R activity and the augmentation of the pituitary weight. (1) Šimić, B., Kniewald, J., Davies, J.E., Kniewald, Z. (1991) Bull. Environ. Contam. Toxicol. 46, 92-99.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA