Naslov
Cytogenetic effects of irinotecan on V79 cells

Autori
Kašuba, Vilena ; Rozgaj, Ružica ; Gamulin, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Mjesto i datum
,

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
irinotecan; comet test; micronucleus test; V79 cell line

Sažetak
Irinotecan is a topoisomerase-interactive agent. It has been widely used in the treatment of metastatic colorectal cancer. The genotoxic effect of maximum single dose (700 mg/m2), recommended monotherapy dose, 350, and recommended combined therapy dose-180 mg/m2 was studied on cultured V79 cells. Two and 24 hours treatment of V79 cells with these concentrations of irinotecan resulted in a concentration-dependent increase of micronucleated binucleate cells and slight, but not statistically significant, increase in the alkaline comet assay results. The frequency of MN cells was significantly higher in irinotecan treated cells then of control samples(non-drug treted cells). Mitomycin C (0.5 ug/mL) treated cells were used as a positive control in this study. Irinotecan tretment also caused alteration in the cell proliferation kinetics. This was evidence by the increase in the frequency of mononucleate cells. The statistical significance of the results was determined using one-way ANOVA test. The results of the comet and micronucleus test show statistically significance in the tail lenght and tail moment as well as micronucleus frequency in drug-treated cells with respect to non-drug treted cells.Chromosome aberration analysis showed the induction of large DNA rearrangements, i.e. a lot of chromosome exchanges. Irinotecan treatment also caused a lot of fragmented nuclei in V79 cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA