Naslov
How alpha beta T cells deal with induced TCRalpha ablation

Autori
Polić, Bojan ; Kunkel, Desiree ; Scheffold, Alexander ; Rajewsky, Klaus

Izvornik
Proceedings of the National Academy of Science of the United States of America (0027-8424) 98 (2001), 15; 8744-8749

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
T cells; TCR; Cre/loxP; CD4; CD8; T-cell homeostasis

Sažetak
On deletion of the gene encoding the constant region of the T cell antigen receptor (TCR)alpha chain in mature T cells by induced Cre-mediated recombination, the cells lose most of their TCR from the cell surface within 7-10 days, but some minute amounts of surface bound TCR beta chains are retained for long periods of time. In a situation in which cellular influx from the thymus is blocked, TCR-deficient naive T cells decay over time, the decay rates being faster for CD8+ cells (t1/2= 16 days) than for CD4+ cells (t1/2= 46 days. TCR+ naive cells are either maintained (CD8+) or decay more slowly (CD4+, t1/2 = 78 days). Numbers of TCR-deficient memory T cells decline very slowly (CD8+ cells, t1/2=52 days) or not at all (CD4+ cells), but are at the population level, these cells fail to expand as their TCR+ counterparts do. Together with earlier data on T cell maintenance in environments lacking appropriate major histocompatibility complex antigens, these data argue against the possibility that spontaneous ligand-independent signaling by the alpha beta TCR contributes significantly to T-cell homeostasis.

Izvorni jezik
Engleski

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