Pregled bibliografske jedinice broj: 170961
Intracellular trafficking of cholera toxin
Intracellular trafficking of cholera toxin // Annual meeting, Croatian Immunological Society / Hrvatsko imunološko društvo (ur.).
Rijeka: Hrvatsko imunološko društvo, 2004. (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Intracellular trafficking of cholera toxin
Autori
Blagojević, Gordana ; Mahmutefendić, Hana ; Kučić, Natalia ; Lučin, Pero
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Annual meeting, Croatian Immunological Society
/ Hrvatsko imunološko društvo - Rijeka : Hrvatsko imunološko društvo, 2004
Skup
Annual meeting, Croatian Immunological Society
Mjesto i datum
Opatija, Hrvatska, 08.10.2004. - 10.10.2004
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
cholera toxin; endocytosis; monensin
Sažetak
Objectives It is known that internalization of cholera toxin (CT) depends on caveolar, clathrin and still unknown ways of endocytosis. The aim of our study was to investigate what is the destiny of CT following its internalization. Methods of study In order to investigate intracellular trafficking of CT we used its B subunit (CTB) labeled with FITC, biotin or Alexa fluor 555. Following endocytosis, surface and intracellular CTB was detected by flow cytometry and immunofluorescence microscopy during the specific time periods. A panel of chemical inhibitors of endocytosis and vesicular transport was used in order to dissect CT intracellular pathway. Furthermore, we used markers of subcellular compartments to detect intracellular localization of CT. Results CTB-Alexa fluor conjugate (CTB-AF555) was localized in Golgi compartments after 30 minutes of incubation at 37 °C and it disappeared from these compartments after 45. During the whole time of the same experiment (45 minutes) we did not colocalized CTB AF555 with lamp-1 (a lysosomal marker). Filipin (an inhibitor of caveolar endocytosis) and monensin (an inhibitor of vesicular acidification) prevented transport of CTB from plasma membrane to Golgi as well as its degradation, while leupeptin (an inhibitor of proteolytic degradation in lysosomes), nocodazol and cytohalazin D (inhibitors of cytoskeletal network) had only partial effect. Conclusions From these results we concluded that CT internalization and intracellular trafficking depends on intact plasma membrane, cytoskeleton and endosomal network.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
