Naslov
Conditional Ablation of NKG2D Receptor in Mouse

Autori
Zafirova, Biljana ; Antulov, Ronald ; Andreanszky, Tibor ; Polić, Bojan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of the Croatian immunological society 2004 / Jonjić Stipan - Rijeka : Hrvatsko imunološko društvo, 2004

Skup
Annual meeting of the Croatian immunological society 2004

Mjesto i datum
Opatija, Hrvatska, 08.10.2004. - 10.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
NKG2D; NK cells; loxP/Cre recombination system; EGFP

Sažetak
NKG2D is an activating receptor expressed on NK cells, activated T cells and macrophages. It belongs to the C-type lectin-like family of receptors and interacts with H60, Rae1 and Mult1 ligands. The aim of this work is to inactivate mouse NKG2D receptor in a conditional and specific manner by the loxP/Cre recombination system in order to study its role in the development, activation, and homeostasis of the NKG2D expressing cell types in vivo. Therefore, we are developing a conditionally NKG2D mutated mouse strain by gene targeting. Taking in account that NKG2D is in some cases expressed only on subpopulations of cells (i.e. activated T cells and macrophages) we are also developing another mouse strain with the knock in of the EGFP gene into the NKG2D locus in order to trace the NKG2D expression. By generating a mouse having both alleles (NKG2D floxed and EGFP knock in/NKG2D knock out) together, we will be able to discriminate conditionally NKG2D deleted from non-deleted cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA