Naslov
Spatio-Temporally Controled Expression Of Cre In T Cells

Autori
Antulov, Ronald ; Zafirova, Biljana ; Polić, Bojan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of the Croatian immunological society 2004 / Jonjić, Stipan - Rijeka : Hrvatsko imunološko društvo, 2004

Skup
Annual meeting of the Croatian immunological society 2004

Mjesto i datum
Rijeka, Hrvatska, 08.10.2004. - 10.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
CD3 epsilon; CreERT2; inducible and T cell specific expression of Cre

Sažetak
T cell antigen receptor (TCR) and pre-TCR complexes are composed of heterodimers in association with dimers of signal transducing invariant subunits (CD3γ , -δ , -ε and ζ ). Inducible DNA deletion between two LoxP sites can be achieved in the mouse using Cre-ERT2, a fusion protein between a mutated ligand binding domain of the estrogen receptor (ER) and the Cre recombinase, the activity of which can be induced by Tamoxifen (Tam) but not natural ER ligands. To study the role of specific genes involved in activation and homeostasis of T cells in vivo we are developing a mouse strain with the inducible and T cell specific expression of Cre. The specificity and inducibility of Cre will be achieved by inserting a CreERT2 expression cassette under the transcriptional control of CD3ε chain promoter.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA