Naslov
The effect of oil industry high density brines on duckweed Lemna minor L.

Autori
Tkalec, Mirta ; Vidaković-Cifrek, Željka ; Regula, Ivan

Izvornik
Chemosphere (0045-6535) 37 (1998), 13; 2703-2715

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lemna minor L.; calcium chloride; calcium bromide; high density brines; toxicity evaluation

Sažetak
Duckweed Lemna minor L. is a suitable plant model for toxicity evaluation of many substances due to its small size, rapid growth and ease of culture. Saturated water solutions of calcium chloride and calcium bromide and their 1:1 mixture are commonly used as high density brines for pressure control in oil wells. These solutions were added in Hoagland's nutrient medium in amount appropriate to achieve 0.5%, 1.0%, 1.5% and 2.0 (v/v) dilutions and after two weeks of exposure the effect of tested chemicals on growth was estimaded by counting fronds, measuring fresh and dry weights and determining total surface area of plants. Chlorophyll and carotenoid content in Lemna minor was also measured. Additionally, anthocyanin content in Spirodela polyrrhiza (L.) Schleiden was determined. During 14 days of exposure tested chemicals in lower concentrations (0.5%, 1.0% and 1.5% v/v) promoted the growth of Lemna minor, but they inhibited it in the highest (2.0% v/v). With increased concentration of tested solutions the concentrations of chlorophyll a and chlorophyll b were correspondingly higher in comparison with the control. Total carotenoid content and chl a/chl b ratio were also increased. The highest anthocyanin content in lower epidermis of Spirodela polyrrhiza was noticed after the treatment with media containing 2.0% (v/v) CaCl2 and 1:1 mixture of CaCl2 and CaBr2, but lower concentrations of all three tested solutions also resulted in anthocyanin content increase.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA