Naslov
Inhibition of melanoma b16-f10 growth by lipid peroxidation product 4-hydroxynonenal

Autori
Žarković, Neven ; Tillian, M.H. ; Schaur, J. ; Waeg, G. ; Jurin, M. ; Esterbauer, H.

Izvornik
Cancer Biotherapy (1062-8401) 10 (1995), 2; 153-156

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Melanoma B16; Growth inhibition; 4-hydroxynonenal; Oxygen free radicals; Lipid peroxidation

Sažetak
Since a gradual benign-to-malignant progression of murine melanoma B16 after exposure in vitro to hypoxia was described recently, the aim of this study was to test if exposing melanoma B16-F10 cells to aldehyde 4-hydroxynonenal (HNE), which is considered not only as one of the major ''second toxic messengers'' of oxygen free radicals (or oxidative stress), but as a normal constituent of many cells and tissues, might have opposite effects. Treatment of the tumor cells with 50 mu M HNE in vitro or in vivo did not prevent development of the tumors, but inhibited their growth. Tumor growth inhibition was equal for in vitro and in vivo treatment, but appeared after a delay of almost one week since there was no difference of the tumor volume to the control observed during the initial period of the tumor growth. Similarly, both HNE treatment of the tumor cells before transplantation and HNE treatment of the melanoma bearing mice resulted in equally prolonged survival time. Thus, the results obtained suggest that while hypoxia could increase the malignancy of the murine melanoma cells, exposing these cells to one of the major ''second toxic messengers'' of oxygen free radicals, HNE, has almost opposite effects and further indicate the possible use of the aldehyde in vivo.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

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