Pregled bibliografske jedinice broj: 16905
Importance of hydrophilic polysaccharide capsule for marine bacterial adhesion to n-alkane phase
Importance of hydrophilic polysaccharide capsule for marine bacterial adhesion to n-alkane phase // Limnology and Oceanography: Navigation into the Next Century, abstract book / the American Society of Limnology and Oceanography (ur.).
Waco (TX): the American Society of Limnology and Oceanography, 1999. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 16905 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Importance of hydrophilic polysaccharide capsule for marine bacterial adhesion to n-alkane phase
Autori
Ivošević, Nadica ; Baldi, Franco ; Minacci, Andrea ; Pepi, Milva ; Svetličić, Vesna ; Žutić, Vera
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Limnology and Oceanography: Navigation into the Next Century, abstract book
/ The American Society of Limnology and Oceanography - Waco (TX) : The American Society of Limnology and Oceanography, 1999
Skup
Limnology and Oceanography: Navigation into the Next Century
Mjesto i datum
Santa Fe (NM), Sjedinjene Američke Države, 01.02.1999. - 05.02.1999
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
bacterial adhesion; bacterial polysacharide; biodegradation; marine environment
Sažetak
Different modes of cells adhesion to droplets of diesel fuel (mixture of n-alkanes: C12-C28) and n-hexadecane are identified for two Acinetobacter strains. Hydrophobic strain RAG-1 is known to excrete the lipopolysaccharide emulsan, whereas the newly isolated species VE-C3 (Baldi et al. 1997, Di Cello et al.1997) is hydrophilic due to the polysaccharide capsule production. The fluorescence distribution of glucose or mannose in the microbial capsule was observed using molecular probes such as lectine concanavalin-A conjugated with fluoresceine isothiocyanate and confocal laser microscopy (SCLM). By using the dropping mercury electrode as in situ adhesion sensor, quantitative differences were found in biofilm formation, as well as in the mode of oil uptake. Microscopic investigations under SCLM and also under TEM by using concanavaline-A conjugated with ferritine clearly demonstrated the incorporation of n-alkane as nanodroplets into polysaccharide matrix of the capsule. The formation of a hydrophobic oil/polysaccharide nanocomposite sustains a continuous growth of VE-C3 biomass in oil degrading cultures over a prolonged period of time. These studies are extended to oil incorporation into polysaccharide gels (mucilage) during the massive macroaggregation phenomenon in the Northern Adriatic.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
