Pregled bibliografske jedinice broj: 168886
Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis
Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis // Lab on a chip, 5 (2005), 3; 298-307 (međunarodna recenzija, članak, znanstveni)
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Naslov
Thin chip microsprayer system coupled to
quadrupole time-of-flight mass spectrometer for
glycoconjugate analysis
Autori
Zamfir, Alina ; Lion, Niels ; Vukelić, Željka ; Bindila, Laura ; Rossier, Joel ; Girault, H. Hubert ; Peter-Katalinić, Jasna
Izvornik
Lab on a chip (1473-0189) 5
(2005), 3;
298-307
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
chip microsprayer system ; quadrupole time-of-flight mass spectrometer ; glycoconjugates
Sažetak
A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
