Naslov
CURCUMIN INTERACTS WITH INDUCTION OF UROKINASE-TYPE PLASMINOGEN ACTIVATOR (UPA)BY THE ALKILATING AGENT MNNG

Autori
Sorić, Jasna ; Lončarek, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Genes and Environment: Bridging the gap / Jan van Benthem - Maastricht, 2004, 52-52

Skup
European Enviromental Mutagen Society 34th Annual Meeting

Mjesto i datum
Maastricht, Nizozemska, 04.09.2004. - 08.09.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
.

Sažetak
We have demonstrated that the uPA gene is transcriptionaly induced by MNNG, starting 24 hours post treatment. ERK MAP kinase specific inhibitor did not change neither constitutive neither basal uPA enzymatic activity, while p38 MAP kinase inhibitor could partially abolish the uPA induction. Curcumin appeared as most potent inhibitor of uPA induction, decreasing basal level of uPA and abolishing the inducible effect of MNNG. Curcumin addition and removal from the cellular media at different time points after MNNG treatment, showed that for suppression of uPA by curcumin, the most relevant fact was its constant presence in the growing medium. Furthermore we observed its reversible effect, since curcumin removal from the cell culture media aborted its inhibiting ability. It indicated existence of an persistent signal triggered by MNNG inducing uPA expression and its inhibition by curcumin. Aditionally, here we show that curcumin influences cellular proliferation and disturbs the action of btubulin, as showed by immunostaining. Aldough the precize mehhanism of curcumin action is currently unknown, the results demonstrated, among possible other events, its additional action upon microtubular organization and consequent induction of mitotic catastrophe (micronucleation).

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA