Naslov
Effect of transduced protein on the intracellular events

Autori
Grdiša, Mira

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS'03 / - Brisel, 2003

Skup
FEBS Meeting on Signal Transduction

Mjesto i datum
Bruxelles, Belgija, 03.07.2003. - 08.07.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cell cycle; regulatory proteins; protein transduction

Sažetak
The plasma membrane of mammalian cells is one of the tight barriers against gene or protein transfer. The potential for intracellular therapeutic use of proteins, peptides and oligonucleotides has been limited by the impermeable nature of the cell membrane to these compounds. To achieve an efficient intracellular drug and DNA delivery, attempts were made to target microparticulate drug carriers into cytoplasm bypassing the endocytotic pathway. TAT peptides derived from the HIV-1 TAT protein facilitate intracellular delivery of proteins and small colloidal particles. TAT protein enters into the cells when added to the surrounding media. In this study, the effect of transduce proteins on regulation of cell cycle, were examined. By transduction of the proteins responsible for cell cycle regulation (p27, p23, Mp27) the proliferation of different cell lines (NALM, MOLT, Raji, SuDHL, and K562) were measured. A transduced p27 did not remarkable influence on proliferation of examined cell lines. Mutated p27 inhibited the proliferation of examined cell lines up to 30 %. On the other hand, a transduction of p23 protein, truncated form of p27, inhibited the proliferation all of examined cell lines 30-60 %.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA