Naslov
Optički senzori za mjerenje antioksidativnog kapaciteta bazirani na immobiliziranim kromogenim radikalima
(AN OPTICAL SENSOR FOR ANTIOXIDATIVE CAPACITY BASED ON IMMOBILISED CHROMOGENIC RADICALS)

Autori
Murković Steinberg, Ivana ; Milardović, Stjepan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Seventh European Conference on Optical Chemical Sensors and Biosensors, EUROPT(R)DE VII, Madrid, April 4-7, 2004. Seventh European Conference on Optical Chemical Sensors and Biosensors, EUROPT(R)DE VII, Madrid, April 4-7, 2004. Book of Abstract / Orellana, Guillermo - Madrid, 2004

Skup
Seventh European Conference on Optical Chemical Sensors and Biosensors, EUROPT(R)DE VII

Mjesto i datum
Madrid, Španjolska, 04.04.2004. - 07.04.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidant activity; polyphenols; DPPH radical; plasticized PVC; optical sensors

Sažetak
Understanding the chemistry of free radicals and antioxidants is gaining importance, particularly in the areas of clinical medicine and nutrition science. Free radicals are known to cause damage to lipids, proteins and nucleic acids and hence are implicated in a number of pathological interactions and degenerative diseases in living organisms, including cancer, Alzheimer’ s and Parkinson’ s disease. Antioxidants act as free radical scavengers and can prevent the damage caused by oxidative reactions. Hence, the evaluation of the antioxidative activity of biomedical or food samples provides useful clinical and dietary information. A number of different optical-based tests for the determination of antioxidant capacity have been previously devised using a variety of chemical and biological mechanisms. In general, the activity of antioxidants present in the sample is related to their ability to scavenge long-living chromogenic or fluorogenic free radicals. Some of these optical assays have been further adapted for use in commercial test kits and instruments using solution-based chemistries. The aim of this work is to study the feasibility of developing an optical sensor test strip having an immobilised chemistry format with which to assess the antioxidative capacity of samples. For this purpose, a number of stable chromogenic radicals, including 2, 2’ -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS• +), 2, 2-diphenyl-1-picrylhydrazyl (DPPH• ) and 2, 6-di-tert-butyl-α -(3, 5-di-tert-butyl-oxo-2, 5-cyclohexadien-1-ylidene)-p-tolyloxy (galvinoxy free radical), were immobilised into a polymer matrix and screened for their suitability as indicators of antioxidative status. The spectrophotometric characterisation of polymer films containing immobilised free radicals was performed, and the effects of the immobilisation of the free radicals on their stability and reactivity towards standard antioxidants were studied.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA