Naslov
BHK 21 C13 cells for Aujeszky's disease virus production with multiple harvest process

Autori
Gaurina Srček, Višnja ; Čajavec, Stanislav ; Sladić, Davor ; Kniewald, Zlatko

Izvornik
Cytotechnology (0920-9069) 45 (2004), 3; 101-106

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Aujeszky's disease virus; baby hamster kidney cells; biomass production; multiple harvest process; spinner flask

Sažetak
Production of Aujeszky's disease virus (ADV) from BHK 21 C13 suspension cells in a simple harvest and multiple harvest process biomass utilization was examined. We studied growth kinetics of BHK 21 C31 cells in 750 ml spinner flask containing 500 ml of culture medium. In simple harvest process of ADV production, 425 ml of virus harvest was obtained with a virus titer of 106.4 TCID50 ml-1 which corresponds to 10676 doses of vaccine. Multiple harvest process resulted in 850 ml of virus harvest, a virus titer of 106.5 TCID50 ml-1 and 26877 AD vaccine doses. In conclusion, multiple harvest process of BHK 21 C13 biomass utilization can be considered as a favorable process to produce AD virus.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA