Naslov
Examination of cytotoxicity and mutagenicity of the water extracts of the coagulant and its waste by-product

Autori
Durgo, Ksenija ; Oreščanin, Višnja ; Garaj-Vrhovac, Verica ; Franekić Čolić, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CROTOX 2004 3 rd Croatian Congress of Toxicology (with international participation) / - Zagreb, 2004

Skup
CROTOX 2004 3 rd Croatian Congress of Toxicology (with international participation)

Mjesto i datum
NP Plitvička jezera, Hrvatska, 26.05.2004. - 29.05.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
heavy metals; Salmonella typhimurium; micronucleus test

Sažetak
Heavy metals from industrial effluents present a dangerous contaminant of agricultural soil and drinking water. After thier introducing into the environment, heavy metals enter a food chain and cause health disturbance in plants and animals. In order to decrease leaching of heavy metals from industrial waste waters highly enriched in heavy metals, especially Cu, Zn and Pb, a new coagulant was produced. The aim of this work was the toxicological characterization of the coagulant and its by-product (i.e. waste mud) remained after the waste water cleaning with the coagulant. Also, metal salts and their mixture were tested in the concentrations that represent maximum allowed values for water extracts of technological waste. For determination of cytotoxicity and abillity of investigated compounds to cause point mutations, two strains of bacteria Salmonella typhimurium, TA98 and TA100 were used. Cytogenetic investigations were preformed on human peripheral blood lymphocytes. Two standard cytogenetic methods were used: micronucleus assay and study of in vitro cell proliferation kinetics (expressed as mitotic index). Water extracts of the coagulant and its waste mud did not induce significant increase of the micronuclei in human peripheral blood lymphocytes. They also did not disturb lymphocyte proliferation kinetics in vitro. As regards lead nitrite it proved not to be cytotoxic on bacterial strains in the tested concentration range (1-100 mg/mL), whereas zinc chloride showed cytotoxic effect for the concentrations above 25 mg/mL. The Ames test results were negative for the nontoxic concentrations of these metals. Only in the blood samples treated with 100 mg/L of zinc chloride, as well as with 10mg/L and 100mg/L of lead nitrate there was significantly higher number of the micronuclei compared to the controls. Similar results were obtained on the basis of the mitotoc index. The results shown that zinc and lead concentrations in the coagulant and waste mud extracts were lower than those that caused significant genotoxic effect on used test system.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

POVEZANOST RADA